AAV Vector Service
The adeno-associated virus (AAV) core facility, operating on a fee-for-service basis, is primarily a resource for CBMM investigators and investigators within the Rutgers community.  The AAV Core provides custom AAV production in 1, 2, 8, and 9 serotypes for applications for basic and pre-clinical studies. If you need other serotypes, please inquire.

Our production method utilizes a triple transfection protocol in HEK293 cells and is purified by Iodixanol gradient purification. The final purified virus particles are serum-free and antibiotic-free, with few empty particles, so that the purified virus particles are suitable for in vivo injection studies.

For a standard preparation, we guarantee a titer of 1 x 10¹¹, although average yield is usually 1 x 10¹² virus particles. Approximate turnaround time is 3-4 weeks.

The cost of rAAV production
Cost for the transfection, AAV production and purification of rAAV ready for animal injection studies, ~500 µl (~1x10⁹ virus particles/ µl)

Members within the CBMM department:       $1,500

NJMS, Rutgers members:                             $3,000

Additional services

Vector cloning:                                             $1,500

Large Scale plasmid preparation                    $250

Please inquire Dr. Gopal J. Babu (babugo@njms.rutgers.edu) for pricing for other services including higher viral titer, crude AAV preparation or AAV purification alone.
The order form and purified plasmid construct (~300 µg for a standard preparation) should be downloaded, filled out, and submitted to:

Dr. Gopal J Babu/ Dr. Yimin Tian
AAV Core Facility
MSB G-630
Department of Cell Biology and Molecular Medicine
NJMS, Rutgers