Proteomics and Bioinformatics Research

The Center for Advanced Proteomics Research (CAPR) is located at Rutgers Biomedical and Health Sciences-New Jersey Medical School in Newark, NJ. We are equipped with state-of-the-art proteomics instruments and bioinformatics systems, including one Thermo Electron Orbitrap Fusion Lumos Tribrid and two Thermo Scientific Q Exactive mass spectrometers, each coupled with a Dionex UltiMate 3000 RSLCnano system. With these advanced instruments, our team of experienced and responsive scientists have contributed to the success of researchers at Rutgers, in the NY area and from the world. We welcome new customers, collaborators and joint grant applications.

We offer comprehensive services for protein analysis at both cell and tissue levels in the context of disease development and regulation, featuring the most advanced proteomics technologies currently available, including

1) Protein identification and characterization;

2) Identification of post-translational modifications (PTMs), e.g. phosphorylation, nitrosylation, acetylation, methylation and disulfide bonds, etc.;

3) Global quantitation of proteins, e.g. Protein-protein interaction using label free quantitation, Differential protein expression using iTRAQ or TMT labeling, Global PTM quantitation using SILAC, iodo-TMT.

We have continuously focused on developing novel proteomics methods to study redox-signaling modifications, including s-nitrosylation, s-sulfonation, and disulfides. In addition, we have developed and optimized state-of-the-art proteomics approaches for global protein expression, protein PTMs and protein-protein interaction studies. Many of these are collaborative studies enabled the discovery of novel mechanistic insights in models of human diseases.

Please contact Dr. Hong Li to discuss your potential proteomics projects.

Recent Publications

2020

  • Xiaowen Wang, Jun Yang, Justin Wong, Jessie Yanxiang Guo, Holly Van Remmen, Hong Li, Eileen White, Chen Liu, Megerditch Kiledjian and X.F. Steven Zheng, SOD1 Is Crucial for Growth and Ribosome Biogenesis in KRAS-p53 Driven Lung Tumors in Mice. Submitted to Nature Communications
  • Baljinnyam E., Venkatesh S., Tong M., Yan L., Liu T., Li H., Xie L.-H., Suzuki C., Fraidenraich D., and Sadoshima J. Proteomic analysis of mitochondrial biogenesis in cardiomyocytes differentiated from human induced pluripotent stem cells. Submitted.
  • Yadaw, A., Siddiq, M., Tolentino, R., Rabinovic,h V., Jayaraman, G., Jain, M., Liu, T., Li, H., Goldfarb, J., Iyengar, R., Hansen, J. (2019) Whole cell response to receptor stimulation involves many deep and distributed subcellular processes. Submitted to Bioinformatics.

 

2019

 

2018

2017


2016

 

2015

  • Fang Zhong, Haibing Chen, Evren U. Azeloglu Chengguo Wei , Weijia Zhang , Zhengzhe Li , Peter Y. Chuang , Belinda Jim, Hong Li, Hongyu Chen, Yongjun Wang, Weiping Jia, Kyung Lee, and John Cijiang He.(2015) Protein S Protects Against Podocyte Injury in Diabetic Nephropathy, JCI. submitted

2014

  • Grant, J. E., Li, H., (2014). Identifying citrullination sites by mass spectrometry. In Protein Deimination in Human Health and Disease, Vol XIII, Springer, New York, Nicholas, A., Bhattacharya, S. (Ed)

 

2013

2012

2011

2010

  • Oka, S.-I., Ago, T., Liu, T., Li, H., Kitazono, T., Sadoshima, J., (2010). Redox Regulation of Class II Histone Deacetylases. in Methods in Redox Signaling (chap. 26), Das, D. (Ed.), Mary Ann Liebert, Inc., New Rochelle. p. 201-206

2009

  • Liu, T., Hu, J., Li, H., in: Ottens, A. K., Wang, K. K. W. (Eds.), iTRAQ-Based Shotgun Neuroproteomics, Neuro Proteomics, Methods in Molecular Biology , Humana Press, Totowa 2009, p. 322.

2008

2007

2006

2005

2004

2003-Before