Peptide HPLC: Analytical/Without Fraction Collection
Automated peptide mapping by C-18 reverse-phase HPLC is available
on a DX 500 (DIONEX) system. Digests are loaded in 50 µl of
a suitable solvent, (which should not contain detergent) and should
contain from 5 to 500 pmol protein. Unless otherwise requested, peptides
will be detected by their absorbance at 220 nm.
Peptide HPLC: Preparative/With Fraction Collection
Protein digests that contain from 5 to 500 picomoles in a volume
of 50 µl may be fractionated by C-18 reverse-phase HPLC collected
fractions . If further purification is required, individual peaks
collected from an HPLC collection run of a protein digest can be re-chromatographed
on C-18 reverse phase.
