Luminex:Luminex® xMAP® technology offers ideal speed and sensitivity for performing multiplexed cytokine measurements. Luminex’s approach offers comparable sensitivity to traditional ELISA-based systems, but with additional advantages including extended dynamic range and smaller sample size.Luminex 100 IS system is developed based on xMAP® technology and it is a flexible analyzer based on the principles of flow cytometry. The system enables to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. This gives opportunities for performing quick and cost-effective bioassays, while ensuring optimum accuracy. The Luminex 100 IS System is the combination of three core xMAP technologies. The first is xMAP microspheres, a family of 100 fluorescently dyed 5.6 micron-sized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. The second is a flow cytometry-based instrument, the Luminex 100, which integrates key xMAP detection components such as lasers, optics, advanced fluidics and high-speed digital signal processors. The lasers excite the internal dyes that identify each microsphere particle, and also any reporter dye captured during the assay. The third component is the IS 2.3 software, which is designed for template-based data acquisition with robust data regression analysis.

The Luminex fluorescent microsphere sorter is a good platform for medium throughput genotyping projects, capable of analyzing anywhere from one SNP in one individual up to 50 SNPs in unlimited numbers of individuals. This device analyzes beads labeled with varying ratios of two fluorescent dyes, and can resolve 100 different dye sets. The fluorescent microspheres used in the SNP assay are coupled to oligonucleotides (the "TAG sequence") such that each particular bead's fluorescent address is associated with a known 20 nucleotide sequence. User oligos, comprised of a SNP specific sequence coupled to the complement of the bead-associated oligo sequence, are synthesized and used in allele specific extension protocols with biotinylated nucleotides. Upon incubation with strepavidin-phycoerythrin (SA-PE), labeled SNP allele-specific extension products are created. These products are combined with selected fluorescent bead-oligo combinations, then sorted and quantified on the Luminex instrument; this results in an amount of PE signal associated with a particular fluorescent bead address. Each address is linked to a particular oligo which in turn is linked to a particular SNP based on the experimental design. Thus, differences in signal intensity determine which alleles are present in a given input PCR fragment.

STANDARD PRICES:

Charge per run $25.00