Wednesdays, 1:00-3:00 PM
Credits: 2
Grade Mode: Normal
Start Date: 09/03/2025
End date:12/17/2025
Final Exam Date: 12/17/2025
Instructor: Kabilan Velliya Gounder
Course Description:
Methods in Microscopic Imaging is a comprehensive two-semester course designed to provide students with both theoretical knowledge and optional hands-on training in contemporary microscopic techniques. The course covers the principles and applications of light microscopy, fluorescence microscopy, laser capture microdissection, and scanning electron microscopy. Students will develop an in-depth understanding of image formation, resolution, optical contrast methods (such as phase contrast and differential interference contrast), as well as the use of fluorophores and digital imaging systems. Advanced topics include live-cell imaging and fluorescence-based assays. The course also introduces students to quantitative image analysis using software such as ImageJ and discusses common imaging artifacts and troubleshooting strategies. Optional hands-on laboratory sessions are integrated throughout the course to reinforce learning and provide exposure to real-world imaging applications. All lecture materials will be shared in advance, and students are expected to review them before class. The final grade will be based on a comprehensive written exam, which will include a combination of multiple-choice questions and open-ended questions designed to assess both conceptual understanding and analytical skills.
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