Proteomics and Bioinformatics Research

The Center for Advanced Proteomics Research (CAPR) is located at Rutgers Biomedical and Health Sciences-New Jersey Medical School in Newark, NJ. We are equipped with state-of-the-art proteomics instruments and bioinformatics systems, including a Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer coupled with a Dionex UltiMate 3000 RSLCnano system. With this advanced instrument, our team of experienced and responsive scientists have contributed to the success of researchers at Rutgers, in the NY area and from the world. We welcome new customers, collaborators and joint grant applications.

We offer comprehensive services for protein analysis at both cell and tissue levels in the context of disease development and regulation, featuring the most advanced proteomics technologies currently available, including

1) Protein identification and characterization;

2) Identification of post-translational modifications (PTMs), e.g. phosphorylation, nitrosylation, acetylation, methylation and disulfide bonds, etc.;

3) Global quantitation of proteins, e.g. Protein-protein interaction using label free quantitation, Differential protein expression using iTRAQ or TMT labeling, Global PTM quantitation using SILAC, iodo-TMT.

We have continuously focused on developing novel proteomics methods to study redox-signaling modifications, including s-nitrosylation, s-sulfonation, and disulfides. In addition, we have developed and optimized state-of-the-art proteomics approaches for global protein expression, protein PTMs and protein-protein interaction studies. Many of these are collaborative studies enabled the discovery of novel mechanistic insights in models of human diseases.

Please contact Dr. Hong Li to discuss your potential proteomics projects.

Recent Publications



  • Cui C, Wu C, Shu P, Liu T, Li H, Beuve A. Soluble guanylyl cyclase mediates noncanonical nitric oxide signaling by nitrosothiol transfer under oxidative stress. Redox Biol. 2022 Aug 2;55:102425. doi: 10.1016/j.redox.2022.102425. Epub ahead of print. PMID: 35961098; PMCID: PMC9372771.

  • Nasuhidehnavi A, Zhao Y, Punetha A, Hemphill A, Li H, Bechtel TJ, Rager T, Xiong B, Petrou VI, Gubbels MJ, Weerapana E, Yap GS. A Role for Basigin in Toxoplasma gondii Infection. Infect Immun. 2022 Aug 18;90(8):e0020522. doi: 10.1128/iai.00205-22. Epub 2022 Aug 1. PMID: 35913173; PMCID: PMC9387297.

  • Du S, Wu S, Feng X, Wang B, Xia S, Liang L, Zhang L, Govindarajalu G, Bunk A, Kadakia F, Mao Q, Guo X, Zhao H, Berkman T, Liu T, Li H, Stillman J, Bekker A, Davidson S, Tao YX. A nerve injury-specific long noncoding RNA promotes neuropathic pain by increasing Ccl2 expression. J Clin Invest. 2022 Jul 1;132(13):e153563. doi: 10.1172/JCI153563. PMID: 35775484; PMCID: PMC9246381.

  • Murari A, Goparaju NSV, Rhooms SK, Hossain KFB, Liang FG, Garcia CJ, Osei C, Liu T, Li H, Kitsis RN, Patel R, Owusu-Ansah E. IDH2-mediated regulation of the biogenesis of the oxidative phosphorylation system. Sci Adv. 2022 May 13;8(19):eabl8716. doi: 10.1126/sciadv.abl8716. Epub 2022 May 11. PMID: 35544578; PMCID: PMC9094667.

  • Xiong Y, Liu T, Chen T, Hansen J, Hu B, Chen Y, Jayaraman G, Schürer S, Vidovic D, Goldfarb J, Sobie EA, Birtwistle MR, Iyengar R, Li H, Azeloglu EU. Proteomic cellular signatures of kinase inhibitor-induced cardiotoxicity. Sci Data. 2022 Jan 20;9(1):18. doi: 10.1038/s41597-021-01114-3. PMID: 35058449; PMCID: PMC8776854.

  • Zhou J, Wu Y, Rauova L, Koma G, Wang L, Poncz M, Li H, Liu T, Fong KP, Bennett JS, Kunapuli SP, Essex DW. A novel role for endoplasmic reticulum protein 46 (ERp46) in platelet function and arterial thrombosis in mice. Blood. 2022 Mar 31;139(13):2050-2065. doi: 10.1182/blood.2021012055. PMID: 34752599; PMCID: PMC8972093.


  • Foo TK, Vincelli G, Huselid E, Her J, Zheng H, Simhadri S, Wang M, Huo Y, Li T, Yu X, Li H, Zhao W, Bunting SF, Xia B. ATR/ATM-Mediated Phosphorylation of BRCA1 T1394 Promotes Homologous Recombinational Repair and G2-M Checkpoint Maintenance. Cancer Res. 2021 Sep 15;81(18):4676-4684. doi: 10.1158/0008-5472.CAN-20-2723. Epub 2021 Jul 23. PMID: 34301763; PMCID: PMC8448966.

  • Murari A, Rhooms SK, Garcia C, Liu T, Li H, Mishra B, Deshong C, Owusu-Ansah E. Dissecting the concordant and disparate roles of NDUFAF3 and NDUFAF4 in mitochondrial complex I biogenesis. iScience. 2021 Jul 16;24(8):102869. doi: 10.1016/j.isci.2021.102869. PMID: 34386730; PMCID: PMC8346666.

  • Pan Z, Du S, Wang K, Guo X, Mao Q, Feng X, Huang L, Wu S, Hou B, Chang YJ, Liu T, Chen T, Li H, Bachmann T, Bekker A, Hu H, Tao YX. Downregulation of a Dorsal Root Ganglion-Specifically Enriched Long Noncoding RNA is Required for Neuropathic Pain by Negatively Regulating RALY-Triggered Ehmt2 Expression. Adv Sci (Weinh). 2021 Jul;8(13):e2004515. doi: 10.1002/advs.202004515. Epub 2021 May 14. PMID: 34383386; PMCID: PMC8356248.

  • Venkatesh S, Baljinnyam E, Tong M, Kashihara T, Yan L, Liu T, Li H, Xie LH, Nakamura M, Oka SI, Suzuki CK, Fraidenraich D, Sadoshima J. Proteomic analysis of mitochondrial biogenesis in cardiomyocytes differentiated from human induced pluripotent stem cells. Am J Physiol Regul Integr Comp Physiol. 2021 Apr 1;320(4):R547-R562. doi: 10.1152/ajpregu.00207.2020. Epub 2020 Oct 28. PMID: 33112656; PMCID: PMC8238141.

  • Alapa M, Cui C, Shu P, Li H, Kholodovych V, Beuve A. Selective cysteines oxidation in soluble guanylyl cyclase catalytic domain is involved in NO activation. Free Radic Biol Med. 2021 Jan;162:450-460. doi: 10.1016/j.freeradbiomed.2020.11.001. Epub 2020 Nov 6. PMID: 33161042; PMCID: PMC7889651.

  • Wang X, Zhang H, Sapio R, Yang J, Wong J, Zhang X, Guo JY, Pine S, Van Remmen H, Li H, White E, Liu C, Kiledjian M, Pestov DG, Steven Zheng XF. SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Nat Commun. 2021 Apr 15;12(1):2259. doi: 10.1038/s41467-021-22480-x. PMID: 33859191; PMCID: PMC8050259.


  • Davra, V., Saleh, T., Geng, K., Kimani, S., Mehta, D., Kasikara, C., Smith, B., Colangelo, N., Ciccarelli, B., Li, H., Azzam, E., Kalodimos, C., Birge, RB., Kumar, A., (2020) Cyclophilin A inhibitor Debio-025 targets Crk, reduces metastasis, and induces tumor immunogenicity in breast cancer. Mol Cancer Res Apr 22. pii: molcanres.1144.2019. doi: 10.1158/1541-7786.MCR-19-1144.
  • Marc A. Egerman, Jenny S. Wong, Tian Runxia , Gohar Mosoyan , Kinsuk Chauhan , Joselyn Reyes-Bahamonde , Nanditha Anandakrishnan , Nicholas J. Wong , Emilia Bagiella , Fadi Salem , Kristin Meliambro , Hong Li , Evren U. Azeloglu , Steven G. Coca , Kirk N. Campbell and Leopoldo Raij. (2020). Plasminogenuria is associated with podocyte injury, edema and kidney dysfunction in incident glomerular disease. The FASEB Journal. In Press.
  • Himelman E, Lillo MA, Nouet J, Gonzalez JP, Zhao Q, Xie LH, Li H, Liu T, Wehrens XH, Lampe PD, Fishman GI, Shirokova N, Contreras JE, Fraidenraich D. (2020) Prevention of Connexin43 remodeling protects against Duchenne muscular dystrophy cardiomyopathy. J Clin Invest 130(4):1713-1727.








  • Fang Zhong, Haibing Chen, Evren U. Azeloglu Chengguo Wei , Weijia Zhang , Zhengzhe Li , Peter Y. Chuang , Belinda Jim, Hong Li, Hongyu Chen, Yongjun Wang, Weiping Jia, Kyung Lee, and John Cijiang He.(2015) Protein S Protects Against Podocyte Injury in Diabetic Nephropathy, JCI. submitted


  • Grant, J. E., Li, H., (2014). Identifying citrullination sites by mass spectrometry. In Protein Deimination in Human Health and Disease, Vol XIII, Springer, New York, Nicholas, A., Bhattacharya, S. (Ed)






  • Oka, S.-I., Ago, T., Liu, T., Li, H., Kitazono, T., Sadoshima, J., (2010). Redox Regulation of Class II Histone Deacetylases. in Methods in Redox Signaling (chap. 26), Das, D. (Ed.), Mary Ann Liebert, Inc., New Rochelle. p. 201-206


  • Liu, T., Hu, J., Li, H., in: Ottens, A. K., Wang, K. K. W. (Eds.), iTRAQ-Based Shotgun Neuroproteomics, Neuro Proteomics, Methods in Molecular Biology , Humana Press, Totowa 2009, p. 322.